Spontaneous Exit from Diplotene Arrest in Mammalian Oocytes in vitro: Is It ROS-Mediated?

  • Ashutosh N. Pandey Cell Physiology Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi-221005, UP, India
  • Meenakshi Tiwari Cell Physiology Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi-221005, UP, India
  • Anumegha Gupta Cell Physiology Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi-221005, UP, India
  • Alka Sharma Cell Physiology Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi-221005, UP, India
  • Shilpa Prasad Cell Physiology Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi-221005, UP, India
  • Pramod K. Yadav Cell Physiology Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi-221005, UP, India
  • Anil K. Yadav Cell Physiology Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi-221005, UP, India
  • Devendra K. Pandey Cell Physiology Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi-221005, UP, India
  • Shail K. Chaube Cell Physiology Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi-221005, UP, India
Keywords: Mammalian oocytes; Reactive oxygen species; Redox signaling; Spontaneous meiotic resumption

Abstract

The diplotene arrest is maintained for a long period within the follicular microenvironment of mammalian ovary. Normally, resumption from diplotene arrest occurs in response to pituitary gonadotrophin surge at puberty. However, gonadotrophin-independent meiotic exit from diplotene arrest was reported in rabbit oocytes cultured in vitro and thereafter in several mammalian species. Since no obvious stimulus is required, scientists consider it as spontaneous exit from diplotene arrest that initiates oocyte maturation in vitro. Emerging evidence suggests that in vitro culture conditions do not meet to the follicular microenvironment and result in generation of reactive oxygen species (ROS) in oocytes. A moderate increase of ROS modulates 3′,5′-cyclic adenosine monophosphate (cAMP) as well as Ca2+ levels and initiates downstream pathways to destabilize maturation promoting factor (MPF). MPF destabilization results in meiotic exit from diplotene arrest under in vitro culture conditions. Thus, spontaneous exit from diplotene arrest under in vitro culture conditions in mammalian oocyte is ROS-mediated.

Published
2020-05-28
How to Cite
Pandey, A. N., Tiwari, M., Gupta, A., Sharma, A., Prasad, S., Yadav, P. K., Yadav , A. K., Pandey, D. K., & Chaube, S. K. (2020). Spontaneous Exit from Diplotene Arrest in Mammalian Oocytes in vitro: Is It ROS-Mediated?. Reactive Oxygen Species, 10(28). Retrieved from https://aimsci.com/ros/index.php/ros/article/view/265
Section
Research Highlights