Low-Dose γ-Irradiation Priming of Seeds Alleviates Aluminum-Induced Oxidative Stress and Genotoxicity in the Oil Seed Crop, Niger, Guizotia abyssinica (L.f.) Cass

  • Dambaru Golari Molecular Biology and Genomics Laboratory, Department of Botany, Berhampur University, Berhampur 760007, India
  • A. Venugopal Molecular Biology and Genomics Laboratory, Department of Botany, Berhampur University, Berhampur 760007, India
  • Kamal K. Panda Molecular Biology and Genomics Laboratory, Department of Botany, Berhampur University, Berhampur 760007, India
  • Narasimham L. Parinandi Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Medicine, Davis Heart and Lung Research Institute, Ohio State University College of Medicine, Columbus, OH 43210, USA
  • Brahma B. Panda Molecular Biology and Genomics Laboratory, Department of Botany, Berhampur University, Berhampur 760007, India
Keywords: Aluminum phytotoxicity; Adaptive response; Genotoxicity; Ionizing radiation; Oil seed; Oxidative stress; Seed priming

Abstract

Aluminium (Al3+) stress is a primary factor limiting crop productivity in the acidic soils of the tropical and sub-tropical regions of the world. Seed priming is a viable approach to sustain crop productivity by counteracting environmental stress. In the present study, we tested the efficacy of seed priming with low dose of γ-irradiation (0.01 kGy) in the oil seed crop, Niger, Guizotia abyssinica (L.f.) Cass. as the model plant  against the challenging treatment of  Al3+ (10 mM) at pH 4.5. Replicated experiments in four sets with appropriate negative and positive controls were conducted. Indices of phytotoxicity (seedling growth and cell death), oxidative stress (generation reactive oxygen species, ROS and lipid peroxidation), genotoxicity (mitotic index and frequency, chromosomal aberrations, and micronucleus formation) and the activities of antioxidant enzymes (catalase, superoxide dismutase, guaiacol peroxidase, ascorbate peroxidase and glutathione reductase) in the leaf tissue and root meristems of the germinating seeds were determined. Priming of seeds with γ-irradiation caused significant inhibition of seedling growth marked by mild oxidative stress with little or no genotoxicity. Seeds treated with Al3+ alone revealed significant inhibition of seedling growth marked by significant induction oxidative stress and genotoxicity. However, seeds which were consecutively primed with γ-irradiation and subsequently exposed to Al3+ challenge, exhibited amelioration of oxidative stress and genotoxicity. Overall, the results of the current study demonstrated that γ-irradiation priming of the dry seeds of G. abyssinica invoked an adaptive response through eliciting mild oxidative stress that ameliorated plant tolerance to Al3+ toxicity.

Published
2018-07-01
How to Cite
Golari, D., Venugopal, A., Panda, K. K., Parinandi, N. L., & Panda, B. B. (2018). Low-Dose γ-Irradiation Priming of Seeds Alleviates Aluminum-Induced Oxidative Stress and Genotoxicity in the Oil Seed Crop, Niger, Guizotia abyssinica (L.f.) Cass. Reactive Oxygen Species, 6(16), 276–288. Retrieved from https://aimsci.com/ros/index.php/ros/article/view/148
Section
Original Research Articles